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T-cell receptor variable genes and genetic susceptibility to celiac disease: an association and linkage study.
T-cell receptor variable genes and genetic susceptibility to celiac disease: an association and linkage study.
- Source :
-
Gastroenterology [Gastroenterology] 1993 Dec; Vol. 105 (6), pp. 1790-6. - Publication Year :
- 1993
-
Abstract
- Background: Genetic susceptibility of celiac disease is primarily associated with a particular combination of and HLA-DQA1/DQB1 gene; however, this does not fully account for the genetic predisposition. Therefore, the aim of this study was to examine whether T-cell receptor (TCR) genes may be susceptibility genes in celiac disease.<br />Methods: HLA class II typing was performed by polymerase chain reaction amplification in combination with sequence-specific oligonucleotide hybridization. TCR alpha (TCRA), TCR gamma (TCRG), and TCR beta (TCRB) loci were investigated by restriction fragment length polymorphism analysis.<br />Results: Allelic frequencies of TCRA, TCRG, and TCRB variable genes were compared between patients with celiac disease (n = 53) and control patients (n = 67), and relative risk (RR) estimates were calculated. The RR was 1.67 for allele C1 at TCRA1, 3.35 for allele D2 at TCRA2, 1.66 for allele B2 at TCRG, and 1.35 for allele B at TCRB, showing no significant association. Additionally, linkage analysis was performed in 23 families. The logarithm of odd scores for celiac disease vs. the TCR variable genes at TCRA, TCRG, and TCRB showed no significant linkage.<br />Conclusions: These data suggest that the analyzed TCR variable gene segments V alpha 1.2, V gamma 11, and V beta 8 do not play a major role in susceptibility to celiac disease.
- Subjects :
- Adolescent
Adult
Aged
Base Sequence
Child
Child, Preschool
Female
Genetic Markers
Genetic Predisposition to Disease
HLA-DQ Antigens genetics
HLA-DQ beta-Chains
HLA-DR3 Antigen genetics
Humans
Male
Middle Aged
Molecular Sequence Data
Celiac Disease genetics
Genetic Linkage
Receptors, Antigen, T-Cell, alpha-beta genetics
Receptors, Antigen, T-Cell, gamma-delta genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0016-5085
- Volume :
- 105
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 8253354
- Full Text :
- https://doi.org/10.1016/0016-5085(93)91077-u