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Sensitive and simple bioassay for human tumour necrosis factor-alpha.
- Source :
-
European cytokine network [Eur Cytokine Netw] 1993 Jul-Aug; Vol. 4 (4), pp. 279-83. - Publication Year :
- 1993
-
Abstract
- A simple, sensitive, and specific in vitro bioassay is described for human tumour necrosis factor alpha (TNF-alpha). The system employs the U937 cell line incubated with 10 ng/ml actinomycin D for maximum sensitivity. This method allows the detection of less than 0.6 pg/ml of TNF-alpha and the log dose-response curve is linear at concentrations 0.6-250 pg/ml TNF-alpha. Highly sensitive subclones of U937 were obtained which were capable of detecting as little as 0.1 pg/ml TNF-alpha. Finally, this bioassay demonstrates specificity by discriminating between TNF-alpha and TNF-beta.
- Subjects :
- Biological Assay
Cell Death drug effects
Clone Cells drug effects
Dactinomycin pharmacology
Dose-Response Relationship, Drug
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Humans
Interleukins pharmacology
Leukemia, Promyelocytic, Acute
Lymphoma, Large B-Cell, Diffuse pathology
Lymphotoxin-alpha pharmacology
Recombinant Proteins pharmacology
Sensitivity and Specificity
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1148-5493
- Volume :
- 4
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European cytokine network
- Publication Type :
- Academic Journal
- Accession number :
- 8268418