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Loss of transporter protein, encoded by the TAP-1 gene, is highly correlated with loss of HLA expression in cervical carcinomas.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1994 Jan 01; Vol. 179 (1), pp. 335-40. - Publication Year :
- 1994
-
Abstract
- Malignant tumor cells can escape CD8+ cytotoxic T cell killing by downregulating class I major histocompatibility complex (MHC) expression. Stable class I MHC surface expression requires loading of the heavy chain/light chain dimer with antigenic peptide, which is delivered to class I MHC molecules in the endoplasmic reticulum by the presumed peptide transporter, encoded by the transporter associated with antigen presentation (TAP) 1 and 2 genes. We have investigated whether loss of class I MHC expression frequently observed in different cancers could result from interference with TAP function. A polyclonal antiserum, raised against a bacterial glutathione S-transferase/human TAP-1 fusion protein, was used for the immunohistochemical analysis of TAP-1 expression in 76 cervical carcinomas. Results showed loss of TAP-1 expression in neoplastic cells in 37 out of 76 carcinomas. Immunohistochemical double staining procedures in combination with HLA-specific antibodies revealed congruent loss at the single cell level of TAP-1 and HLA-A/B expression in 28 out of 37 carcinomas. The remaining samples expressed HLA(-A) in the absence of TAP-1 (n = 6) or showed loss of HLA(-A/B) while TAP-1 was expressed (n = 3). These data strongly indicate that inhibition of peptide transport by downregulation of TAP-1 is a potential strategy of malignant cells to evade immune surveillance.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 2
Antibody Specificity
Biological Transport
Carrier Proteins genetics
Female
HLA Antigens genetics
HLA Antigens immunology
Histocompatibility Antigens Class I genetics
Histocompatibility Antigens Class II genetics
Humans
Immune Sera
ATP-Binding Cassette Transporters
Carrier Proteins metabolism
HLA Antigens biosynthesis
Histocompatibility Antigens Class II metabolism
Uterine Cervical Neoplasms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 179
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 8270878
- Full Text :
- https://doi.org/10.1084/jem.179.1.335