Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method.

Authors :: Koide M
Saito A
Kusano N
Higa F
Source :: Applied and environmental microbiology [Appl Environ Microbiol] 1993 Jun; Vol. 59 (6), pp. 1943-6.
Publication Year :: 1993
Abstract: The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.

Subjects :: Bacteriological Techniques
Base Sequence
DNA, Bacterial chemistry
Hospitals
Legionella pneumophila isolation & purification
Molecular Sequence Data
Sensitivity and Specificity
DNA, Bacterial genetics
Legionella isolation & purification
Polymerase Chain Reaction methods
Water Microbiology

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