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Characterization of the family Pasteurellaceae on the basis of cellular lipids and carbohydrates.
- Source :
-
Zentralblatt fur Bakteriologie : international journal of medical microbiology [Zentralbl Bakteriol] 1993 Jun; Vol. 279 (1), pp. 104-13. - Publication Year :
- 1993
-
Abstract
- Selected strains representing established and newly described taxa in the family Pasteurellaceae were investigated for their cellular lipid and carbohydrate composition to clarify the taxonomic significance of such features. Methylated cellular fatty acids and acetylated derivatives of the cellular carbohydrates were determined by capillary gas chromatography using a flame ionization detector. In part the carbohydrates were identified by mass spectrometry. Phospholipids were determined by thin layer chromatography, the lipoquinones by high pressure liquid chromatography. The cellular fatty acid patterns proved to be uniform with minor variations, but the separation from the Neisseriaceae and from Moraxella was possible. Also the distribution of the phospholipids was uniform within the family. The lipoquinone contents were useful for the discrimination of groups within the family not necessarily reflecting the degree of genomic relatedness. The analysis of the cellular carbohydrates resulted in a common sugar pattern with all members of the family and characteristic carbohydrate profiles discriminating groups, often to the species level. All of the cytochemical features considered were useful for the characterization of the family Pasteurellaceae.
- Subjects :
- Actinobacillus chemistry
Actinobacillus classification
Fatty Acids analysis
Haemophilus chemistry
Haemophilus classification
Pasteurella chemistry
Pasteurella classification
Pasteurellaceae chemistry
Phospholipids analysis
Carbohydrates analysis
Lipids analysis
Pasteurellaceae classification
Quinones analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0934-8840
- Volume :
- 279
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zentralblatt fur Bakteriologie : international journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 8369580
- Full Text :
- https://doi.org/10.1016/s0934-8840(11)80496-2