Back to Search
Start Over
Effects of protease inhibitors on binding of sperm to the vitelline coat of ascidian eggs: implications for participation of a proteasome (multicatalytic proteinase complex).
- Source :
-
The Journal of experimental zoology [J Exp Zool] 1993 Sep 15; Vol. 267 (1), pp. 86-91. - Publication Year :
- 1993
-
Abstract
- Among various protease inhibitors, chymostatin (an inhibitor of sperm chymotrypsin-like protease) strongly inhibited the binding of sperm to the vitelline coat of glycerinated eggs of the ascidian Halocynthia roretzi, whereas leupeptin (an inhibitor for sperm acrosin), antipain, and soybean trypsin inhibitor had no significant inhibitory effects. Dansyl-Val-Pro-argininal (an inhibitor of the sperm trypsin-like protease, spermosin) had an inhibitory effect on the binding of sperm that was much smaller than its effects on fertilization. Since the sperm chymotrypsin-like protease that is involved in ascidian fertilization has been identified as a proteasome (multicatalytic proteinase complex), we tested the effects of several peptidyl argininals, inhibitors of the activities of proteasomes, on this binding process. The ranking of the inhibitory effects of these compounds on the binding of sperm was the same as that of their effects on the chymotrypsin-like activity of the proteasome, reported previously. The potent inhibitors of binding used in these studies had no or minimal effects on sperm motility. These results suggest that a sperm chymotrypsin-like protease (most probably the chymotrypsin-like protease in the proteasome) plays a key role in binding of sperm to the vitelline coat of the ascidian egg.
- Subjects :
- Amino Acid Sequence
Animals
Arginine analogs & derivatives
Female
Fertilization physiology
Male
Molecular Sequence Data
Oligopeptides pharmacology
Proteasome Endopeptidase Complex
Sperm Motility
Sperm-Ovum Interactions drug effects
Urochordata
Vitelline Membrane drug effects
Cysteine Endopeptidases physiology
Multienzyme Complexes physiology
Protease Inhibitors pharmacology
Sperm-Ovum Interactions physiology
Vitelline Membrane physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-104X
- Volume :
- 267
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of experimental zoology
- Publication Type :
- Academic Journal
- Accession number :
- 8376953
- Full Text :
- https://doi.org/10.1002/jez.1402670112