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Comparison of receptors for Escherichia coli heat-stable enterotoxin: novel receptor present in IEC-6 cells.
- Source :
-
The American journal of physiology [Am J Physiol] 1993 Jan; Vol. 264 (1 Pt 1), pp. G172-8. - Publication Year :
- 1993
-
Abstract
- Enterotoxigenic Escherichia coli elaborate a heat-stable enterotoxin that causes diarrhea in humans and animals. The primary event in the diarrheal cascade is the binding of this enterotoxin to specific receptors on enterocytes and activation of guanylyl cyclase. Two intestinal cell lines, Caco-2 and IEC-6, were tested for the presence of these receptors. Although both cell lines exhibited specific binding, only the Caco-2 cell line responded to heat-stable enterotoxin with increased guanylyl cyclase activity. Cloning and expression studies confirmed that the receptor present in Caco-2 cells is a homologue of guanylyl cyclase C, a known transmembrane heat-stable enterotoxin receptor. Expression of the receptor in differentiating Caco-2 cells increases with cell maturation, indicating that these cells are a suitable model for future studies. However, Northern and polymerase chain reaction analyses demonstrated that guanylyl cyclase C is not expressed in IEC-6 cells, strongly suggesting the presence of a novel heat-stable enterotoxin receptor that is not coupled to guanylyl cyclase activity.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cloning, Molecular
DNA metabolism
Drug Stability
Hot Temperature
Intestines cytology
Molecular Probes genetics
Molecular Sequence Data
RNA, Messenger metabolism
Receptors, Cell Surface genetics
Receptors, Enterotoxin
Receptors, Guanylate Cyclase-Coupled
Tumor Cells, Cultured
Escherichia coli
Guanylate Cyclase
Intestinal Mucosa metabolism
Receptors, Cell Surface metabolism
Receptors, Peptide
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 264
- Issue :
- 1 Pt 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 8381596
- Full Text :
- https://doi.org/10.1152/ajpgi.1993.264.1.G172