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Identification and characterization of the proBA operon of Streptococcus bovis.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1993 Feb; Vol. 59 (2), pp. 519-22. - Publication Year :
- 1993
-
Abstract
- A genomic DNA library of the rumen bacterium Streptococcus bovis was constructed in Escherichia coli, and recombinant plasmids able to complement proA and proB mutations of the host were found. Southern hybridization and restriction analysis showed that a 3.5-kb fragment of S. bovis DNA contained two genes, organized in an operon and coding for enzymes functionally similar to the glutamyl phosphate reductase-glutamyl kinase enzyme complex that in E. coli catalyzes the first step of proline biosynthesis. Complementation of the E. coli mutations was observed with the fragment inserted in both orientations, which suggested that the S. bovis proBA operon was transcribed from its own promoter. Genetic and biochemical data suggested that the proline biosynthetic pathway of S. bovis is similar to the one previously characterized for E. coli.
- Subjects :
- Gene Library
Oxidoreductases metabolism
Phosphotransferases metabolism
Proline genetics
Streptococcus bovis enzymology
DNA, Bacterial genetics
DNA, Recombinant genetics
Escherichia coli genetics
Operon genetics
Oxidoreductases genetics
Phosphotransferases genetics
Proline biosynthesis
Streptococcus bovis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 59
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 8382022
- Full Text :
- https://doi.org/10.1128/aem.59.2.519-522.1993