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Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia.

Authors :
Bailey AM
Mena GL
Herrera-Estrella L
Source :
Current genetics [Curr Genet] 1993 Jan; Vol. 23 (1), pp. 42-6.
Publication Year :
1993

Abstract

Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

Details

Language :
English
ISSN :
0172-8083
Volume :
23
Issue :
1
Database :
MEDLINE
Journal :
Current genetics
Publication Type :
Academic Journal
Accession number :
8428382
Full Text :
https://doi.org/10.1007/BF00336748