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Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia.
- Source :
-
Current genetics [Curr Genet] 1993 Jan; Vol. 23 (1), pp. 42-6. - Publication Year :
- 1993
-
Abstract
- Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.
Details
- Language :
- English
- ISSN :
- 0172-8083
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 8428382
- Full Text :
- https://doi.org/10.1007/BF00336748