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Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation.
- Source :
-
Fertility and sterility [Fertil Steril] 1993 Apr; Vol. 59 (4), pp. 869-75. - Publication Year :
- 1993
-
Abstract
- Objective: To investigate the ability of N-formyl-methionyl-leucyl-phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients.<br />Design: Prospective, controlled study measuring human spermatozoal reactive oxygen species generation after addition of f-MLP, C5a, or NGF.<br />Setting: A large health maintenance organization.<br />Patients, Participants: The fertile group consisted of 14 men with established fertility and normal bulk semen parameters. The infertile group was comprised of 8 men who were infertile after > 18 months of unprotected sexual intercourse.<br />Interventions: The sperm samples were subjected to four test conditions: f-MLP stimulation, C5a stimulation, NGF stimulation, and no stimulation (control).<br />Main Outcome Measure: Reactive oxygen generation was measured over a 15-minute period using the method of chemiluminescence.<br />Results: In both the fertile and infertile groups, reactive oxygen species generation was significantly enhanced by f-MLP, C5a, and NGF compared with controls. No significant difference in f-MLP- and C5a-stimulated reactive oxygen production was demonstrated between the infertile and fertile groups; however, there was a significant difference in reactive oxygen generation between infertile and fertile subjects when stimulated with NGF.<br />Conclusions: The current study represents the first report of f-MLP-, C5a-, and NGF-stimulated reactive oxygen species generation by human spermatozoa. Nerve growth factor enhanced reactive oxygen species production to a greater extent in infertile subjects compared with fertile subjects. This points to a possible NGF-mediated biochemical defect in the sperm of infertile patients.
Details
- Language :
- English
- ISSN :
- 0015-0282
- Volume :
- 59
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Fertility and sterility
- Publication Type :
- Academic Journal
- Accession number :
- 8458510
- Full Text :
- https://doi.org/10.1016/s0015-0282(16)55874-9