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Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1993 Mar; Vol. 59 (3), pp. 927-32. - Publication Year :
- 1993
-
Abstract
- An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The enzyme exhibited from 45 to 66% identity with five bacterial beta-glucosidases.
- Subjects :
- Amino Acid Sequence
Bacillus genetics
Base Sequence
Cellobiose biosynthesis
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cloning, Molecular
DNA, Bacterial chemistry
Genes, Bacterial
Molecular Sequence Data
Restriction Mapping
Substrate Specificity
beta-Glucosidase chemistry
beta-Glucosidase isolation & purification
beta-Glucosidase physiology
Bacillus enzymology
DNA, Bacterial isolation & purification
beta-Glucosidase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 59
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 8481013
- Full Text :
- https://doi.org/10.1128/aem.59.3.927-932.1993