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Role of intracellular Cd2+ in catecholamine release and lethality in PC12 cells.
- Source :
-
Toxicology letters [Toxicol Lett] 1995 Nov 15; Vol. 81 (2-3), pp. 151-7. - Publication Year :
- 1995
-
Abstract
- To evaluate the role of intracellular Cd2+ in catecholamine release and lethality in rat pheochromocytoma (PC12) cells the following results were obtained: [1] the presence of Cd2+ intracellularly was demonstrated with the Cd(2+)-sensitive fluorescent dye BTC-5N, [2] Cd2+ entry through Ca(2+)-channels was either blocked with nifedipine or diltiazem or increased with Bay K8644, [3] Cd2+ entry through voltage sensitive Ca2+ channels was related to dopamine release and cell lethality, [4] a calmodulin inhibitor protected against Cd2+ toxicity, and [5] extracellular Ca2+ concentration, altered prior to Cd2+ exposure, was inversely related to dopamine release by Cd2+. The data indicate intracellular effects of Cd2+ rather than cell surface actions are primarily involved in neurotransmitter release and lethality by toxic levels of Cd2+ in adrenomedullary cells. To evaluate the role of intracellular Cd2+ in catecholamine release and lethality in rat pheochromocytoma (PC12) cells the following results were obtained: [1] the presence of Cd2+ intracellularly was demonstrated with the Cd(2+)-sensitive fluorescent dye BTC-5N, [2] Cd2+ entry through Ca(2+)-channels was either blocked with nifedipine or diltiazem or increased with Bay K8644, [3] Cd2+ entry through voltage sensitive Ca2+ channels was related to dopamine release and cell lethality, [4] a calmodulin inhibitor protected against Cd2+ toxicity, and [5] extracellular Ca2+ concentration, altered prior to Cd2+ exposure, was inversely related to dopamine release by Cd2+. The data indicate intracellular effects of Cd2+ rather than cell surface actions are primarily involved in neurotransmitter release and lethality by toxic levels of Cd2+ in adrenomedullary cells.
- Subjects :
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester pharmacology
Animals
Calcium Channel Agonists pharmacology
Calcium Channel Blockers pharmacology
Calcium Channels drug effects
Calcium Channels metabolism
Humans
Kinetics
Neurons cytology
Neurons metabolism
PC12 Cells
Rats
Cadmium metabolism
Cadmium toxicity
Cell Death drug effects
Dopamine metabolism
Neurons drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0378-4274
- Volume :
- 81
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 8553369
- Full Text :
- https://doi.org/10.1016/0378-4274(95)03425-0