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Amniochorion: a source of interleukin-8.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 1995 Sep; Vol. 34 (3), pp. 156-62. - Publication Year :
- 1995
-
Abstract
- Problem: Preterm labor and premature rupture of the membranes are major complications of pregnancy. We have reported the possible role of amniochorionic membrane in the production of inflammatory cytokines and the early onset of labor. This study was conducted to detect the expression of IL-8 mRNA and peptide production in cultured fetal membranes.<br />Method: Amniochorionic membranes were collected from women undergoing elective cesarean section at term. Membranes were cultured in an organ explant system and the expression of IL-8 was studied over a 10-day period by RT-PCR and in situ hybridization. IL-8 peptide localization was accomplished using immunocytochemistry.<br />Results: Constitutive expression of IL-8 mRNA in cultured fetal membranes was demonstrated in both amniotic and chorionic leave cells. mRNA and peptide for IL-8 was homogeneously distributed throughout the amniotic and chorionic cells.<br />Conclusion: Human amniochorionic membrane is a source of IL-8 mRNA and peptide.
- Subjects :
- Amnion pathology
Chorion pathology
Female
Fetal Membranes, Premature Rupture genetics
Fetal Membranes, Premature Rupture pathology
Humans
In Situ Hybridization
Interleukin-8 biosynthesis
Interleukin-8 genetics
Organ Culture Techniques
Peptide Biosynthesis
Polymerase Chain Reaction
Pregnancy
RNA, Messenger biosynthesis
Amnion chemistry
Chorion chemistry
Interleukin-8 chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1046-7408
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 8561872
- Full Text :
- https://doi.org/10.1111/j.1600-0897.1995.tb00932.x