Back to Search
Start Over
A natural glycoprotein inhibitor (NIF) of CD11b/CD18 reduces leukocyte adhesion in the liver after hemorrhagic shock.
- Source :
-
Shock (Augusta, Ga.) [Shock] 1995 Sep; Vol. 4 (3), pp. 187-92. - Publication Year :
- 1995
-
Abstract
- This study was designed to assess the effect of neutrophil inhibitory factor (NIF), a novel specific inhibitor of CD11b/CD18 on hepatic leukocyte trafficking by intravital microscopy 5 h after hemorrhagic shock. Anesthetized rats were instrumented for invasive hemodynamical monitoring. Hemorrhagic shock was induced for 60 min by withdrawal of arterial blood (mean arterial blood pressure = 40 mmHg). Rats were adequately resuscitated for 5 h to achieve a mean arterial blood pressure > 100 mmHg and were randomly assigned to blinded treatment with NIF or placebo control protein administered as a single intravenous bolus (10 mg/kg) at the time of resuscitation. Intrahepatic leukocyte adhesion was evaluated by in vivo fluorescence microscopy. There were no significant differences observed in hemodynamic parameters between the shock groups throughout the study, however, NIF significantly reduced firm leukocyte adhesion in liver sinusoids. The results suggest that NIF may be beneficial in the attenuation of the pathological shock-induced leukocyte adhesion.
- Subjects :
- Animals
Cell Adhesion drug effects
Female
Hemodynamics drug effects
Leukocytes metabolism
Leukocytes pathology
Liver drug effects
Liver pathology
Liver Circulation drug effects
Rats
Rats, Sprague-Dawley
Shock, Hemorrhagic pathology
Shock, Hemorrhagic physiopathology
CD18 Antigens drug effects
Glycoproteins therapeutic use
Helminth Proteins therapeutic use
Leukocytes drug effects
Liver blood supply
Macrophage-1 Antigen drug effects
Membrane Proteins
Shock, Hemorrhagic drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1073-2322
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Shock (Augusta, Ga.)
- Publication Type :
- Academic Journal
- Accession number :
- 8574753
- Full Text :
- https://doi.org/10.1097/00024382-199509000-00006