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[Thrombin substrates containing aminobenzoic acids].
- Source :
-
Ukrainskii biokhimicheskii zhurnal (1978) [Ukr Biokhim Zh (1978)] 1995 Jan-Feb; Vol. 67 (1), pp. 57-64. - Publication Year :
- 1995
-
Abstract
- In order to study the interactions of synthetic substrates with the active site of thrombin the compounds of R-CO-Abz-Arg-OMe were obtained which contained, o-, m- and p-amino benzoic acids (Abz) at P2-subsite. R-CO-moiety was butyric, perfluorobutyric, benzoyl, 2-fluorobenzoyl and perfluorobenzoyl groups. Kinetic parameters of their hydrolysis by thrombin were measured and antithrombin activity was studied. Introduction of fluorine atoms into N-acyl groups caused insignificant alterations of the substrate or inhibitor properties of the compounds containing N-butyric group, but significantly influenced the substrate properties, when peptides contained N-benzoyl group. The interaction mechanism of the obtained substrates with thrombin was proposed.
Details
- Language :
- Russian
- ISSN :
- 0201-8470
- Volume :
- 67
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Ukrainskii biokhimicheskii zhurnal (1978)
- Publication Type :
- Academic Journal
- Accession number :
- 8588255