Determination of ipriflavone and its synthetic impurities by high-performance liquid chromatography using diode-array detection.

A simple, accurate and rapid method for the determination of ipriflavone and its synthetic impurities has been developed. It consists in RP-HPLC separation and identification of the impurities from their UV spectra using photodiode-array detection. The method has been validated and shows good specificity, accuracy, precision and sensitivity.