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Detection and sequence analysis of a new herpesvirus-like agent in AIDS and non-AIDS Kaposi's sarcoma in Taiwan.
- Source :
-
Journal of the Formosan Medical Association = Taiwan yi zhi [J Formos Med Assoc] 1996 Jan; Vol. 95 (1), pp. 13-8. - Publication Year :
- 1996
-
Abstract
- A new herpesvirus-like DNA sequence (KSHV) has been recently identified in Kaposi's sarcoma from acquired immunodeficiency syndrome (AIDS) and non-AIDS patients. In order to verify the role of this new viral agent in Taiwan, a series of Kaposi's sarcoma specimens obtained from AIDS and non-AIDS patients in three medical centers representing different geographic locations of Taiwan were surveyed. A total of seven specimens from AIDS patients and 22 specimens from non-AIDS patients were tested for the presence of this herpesvirus-like agent by polymerase chain reaction (PCR) using the published Kaposi's sarcoma 330-233 primers. The tumor DNA was also tested for cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpes-virus type 6 (HHV-6). The Kaposi's sarcoma specimens from AIDS and non-AIDS patients were positive for KSHV except in one case. Only occasional specimens were positive for CMV and EBV, with a slightly higher positive rate for HHV-6 in AIDS and non-AIDS patients. Cloning and sequence analysis of the PCR products from two cases of Kaposi's sarcoma (one each for AIDS and non-AIDS) revealed only minor differences in this DNA sequence, as compared with the published data from north America. These results further support the assumption that this new KSHV is strongly associated with Kaposi's sarcoma in AIDS and non-AIDS patients in Taiwan.
- Subjects :
- Base Sequence
DNA, Viral isolation & purification
Herpesviridae genetics
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Sarcoma, Kaposi etiology
Taiwan
Acquired Immunodeficiency Syndrome complications
DNA, Viral analysis
Herpesviridae isolation & purification
Sarcoma, Kaposi virology
Subjects
Details
- Language :
- English
- ISSN :
- 0929-6646
- Volume :
- 95
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of the Formosan Medical Association = Taiwan yi zhi
- Publication Type :
- Academic Journal
- Accession number :
- 8640089