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Prevention of islet allograft rejection with engineered myoblasts expressing FasL in mice.

Authors :
Lau HT
Yu M
Fontana A
Stoeckert CJ Jr
Source :
Science (New York, N.Y.) [Science] 1996 Jul 05; Vol. 273 (5271), pp. 109-12.
Publication Year :
1996

Abstract

Allogeneic transplantation of islets of Langerhans was facilitated by the cotransplantation of syngeneic myoblasts genetically engineered to express the Fas ligand (FasL). Composite grafting of allogeneic islets with syngeneic myoblasts expressing FasL protected the islet graft from immune rejection and maintained normoglycemia for more than 80 days in mice with streptozotocin-induced diabetes. Graft survival was not prolonged with composite grafts of unmodified myoblasts or Fas-expressing myoblasts. Islet allografts transplanted separately from FasL-expressing myoblasts into the contralateral kidney were rejected, as were similarly transplanted third-party thyroid allografts. Thus, the FasL signal provided site- and immune-specific protection of islet allografts.

Details

Language :
English
ISSN :
0036-8075
Volume :
273
Issue :
5271
Database :
MEDLINE
Journal :
Science (New York, N.Y.)
Publication Type :
Academic Journal
Accession number :
8658177
Full Text :
https://doi.org/10.1126/science.273.5271.109