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Evidence for the early recruitment of T-cell receptor gamma delta+ T cells during rat listeriosis.
- Source :
-
Immunology [Immunology] 1996 Jan; Vol. 87 (1), pp. 21-8. - Publication Year :
- 1996
-
Abstract
- We have previously reported that heat-shock protein (hsp) 60-reactive T-cell receptor (TCR)gamma delta+ T cells appear in the peritoneal cavity during the early stage of infection with Listeria monocytogenes in mice. In this study, we examined the kinetics of TCR gamma delta+ T cells during listeriosis in F344 rats by flow cytometry using a V65 monoclonal antibody (mAb) directed to a constant determinant of rat TCR gamma delta chains. TCR gamma delta+ T cells significantly increased in the peritoneal cavity on day 6 and then decreased by day 10 after infection, in parallel with the kinetics of hsp60 expression in the peritoneal macrophages during listeriosis in F344 rats. Most of the early appearing TCR gamma delta+ T cells were of the CD4- CD8 alpha beta+ CD5+ lymphocyte function-associated antigen (LFA)-1 alpha high CD45RC- interleukin-2 receptor (IL-2R) alpha- phenotype, although a significant fraction of the TCR gamma delta+ T cells expressed CD8 alpha only. The increase in TCR gamma delta+ T cells during listeriosis was prominent in F1 (F344 x Lewis) rats but only marginal in Lewis rats, which was correlated with the expression level of hsp 60 in the peritoneal macrophages. The peritoneal TCR gamma delta+ T cells in naive F344 rats appeared to proliferate significantly in response to recombinant hsp 60 (rhsp 60) derived from Mycobacterium bovis bacillus Calmette-Guérin (BCG). These results imply that the early appearance of hsp 60-reactive TCR gamma delta+ T cells during listerial infection can be generalized across species.
- Subjects :
- Animals
Cell Culture Techniques
Cell Division immunology
Cell Separation
Chaperonin 60 metabolism
Flow Cytometry
Kinetics
Male
Peritoneal Cavity
Rats
Rats, Inbred F344
Rats, Inbred Lew
Chaperonin 60 immunology
Listeriosis immunology
Receptors, Antigen, T-Cell, gamma-delta analysis
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 87
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 8666431