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An ELISA assay for the rapid diagnosis of acute bacterial meningitis.
- Source :
-
Annals of tropical paediatrics [Ann Trop Paediatr] 1995 Dec; Vol. 15 (4), pp. 273-8. - Publication Year :
- 1995
-
Abstract
- A recently introduced ELISA test (EIA-Test) was used for the detection of the common organisms that cause acute bacterial meningitis (ABM). The test--which detects antigens of Haemophilus influenzae type b, Neisseria meningitidis (serogroups A, B, C) and Streptococcus pneumoniae (25 selected types)--is performed in less than 1 hour and read by the naked eye. Cerebrospinal fluid (CSF) specimens of 125 Sudanese children (1 month-14 years) admitted with a provisional diagnosis of meningitis/meningoencephalitis were prospectively examined with the EIA-Test, direct microscopy (DM) using Gram stain and culture. Of the 56 positive CSF specimens, the EIA-Test had the highest yield (100%), followed by culture (33.9%) and DM (30.4%). Nineteen (33.9%) of the positive samples came from children who had been treated with antibiotics prior to admission, and all were positive in the EIA-Test. EIA-Test was also the only positive test in 31 (55.4%) cases. From the present study, and similar to previous observations, the EIA-Test seems to have valuable potential for the rapid diagnosis of ABM and is of particular help in patients who have been pre-treated with antibiotics.
- Subjects :
- Acute Disease
Child
Child, Preschool
Female
Humans
Infant
Male
Meningitis, Bacterial cerebrospinal fluid
Meningitis, Haemophilus diagnosis
Meningitis, Meningococcal diagnosis
Meningitis, Pneumococcal diagnosis
Prospective Studies
Reproducibility of Results
Sudan
Enzyme-Linked Immunosorbent Assay
Meningitis, Bacterial diagnosis
Meningitis, Bacterial microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0272-4936
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Annals of tropical paediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 8687201
- Full Text :
- https://doi.org/10.1080/02724936.1995.11747783