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Methanol traps the troponin-tropomyosin-actin complex in an "off-state".
- Source :
-
Journal of biochemistry [J Biochem] 1995 Dec; Vol. 118 (6), pp. 1293-8. - Publication Year :
- 1995
-
Abstract
- We have investigated the effect of methanol at concentrations below 15% (v/v) on acto-heavy meromyosin (HMM)- and tropomyosin (Tm)-troponin (Tn)-acto-heavy meromyosin-ATPase activities. Methanol slightly enhanced ATPase activity of acto-HMM alone. It had no apparent effect on normalized Tm-Tn-acto-HMM-ATPase activity in the absence of Ca2+. In the presence of Ca2+, however, methanol markedly inhibited normalized Tm-Tn-acto-HMM ATPase activity. These results show that methanol affects the troponin-tropomyosin regulation of acto-HMM ATPase: methanol suppresses the Ca(2+)-sensitivity of the regulatory system and traps it in an "off-state."
- Subjects :
- Actins chemistry
Actins drug effects
Adenosine Triphosphatases drug effects
Animals
Kinetics
Models, Structural
Muscle, Skeletal metabolism
Protein Binding
Rabbits
Tropomyosin chemistry
Tropomyosin drug effects
Troponin chemistry
Troponin drug effects
Actins metabolism
Adenosine Triphosphatases metabolism
Methanol pharmacology
Tropomyosin metabolism
Troponin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 118
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8720149
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a125021