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Comparative efficiency of simple lipopeptide constructs for in vivo induction of virus-specific CTL.

Authors :
Deprez B
Sauzet JP
Boutillon C
Martinon F
Tartar A
Sergheraert C
Guillet JG
Gomard E
Gras-Masse H
Source :
Vaccine [Vaccine] 1996 Apr; Vol. 14 (5), pp. 375-82.
Publication Year :
1996

Abstract

We have previously shown that virus-specific CTL responses can be elicited in vivo by injecting, without adjuvant, 12-40 amino acid-long peptides, modified in C-terminal position by a simple lipidic amino acid. In this paper, we have studied the chemical accessibility, and the ability to induce in mice a CTL response, of a series of lipopeptides derived from the HIV-1 env (312-327) or (302-335) sequences. We showed that a single modification of these peptides by a lipidic amino acid, preferably in C-terminal position, results in the ability to reproducibly induce, without adjuvant, a relevant CTL response. No clear discrimination appeared concerning the nature of the lipidic modification. Our findings indicate that modification of a relatively long peptide by a N epsilon-palmitoyl-L-Lysylamide can be achieved by conventional methods of synthesis and characterization, offering the possibility to develop low-cost synthetic vaccines in models in which the CTL component is of importance.

Details

Language :
English
ISSN :
0264-410X
Volume :
14
Issue :
5
Database :
MEDLINE
Journal :
Vaccine
Publication Type :
Academic Journal
Accession number :
8735547
Full Text :
https://doi.org/10.1016/0264-410x(95)00220-u