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Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola.
- Source :
-
Current genetics [Curr Genet] 1996 Aug; Vol. 30 (3), pp. 251-8. - Publication Year :
- 1996
-
Abstract
- Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting from a single diploid nucleus were determined. A procedure to cross these isolates was developed to investigate the mating system. Three of six crosses were successful, and the segregation of mating types in accordance with the tetrad analysis strongly points to a bipolar heterothallic mating system in M. graminicola. Random ascospore progenies from the successful crosses, each comprising 54 isolates, were studied with three primers to determine the mode of inheritance of the RAPD markers. Mendelian segregation and recombination of RAPD markers was observed in all progenies.
- Subjects :
- Base Sequence
Crosses, Genetic
DNA Primers
Genetic Markers
Microscopy, Electron, Scanning
Molecular Sequence Data
Polymorphism, Genetic
Spores, Fungal ultrastructure
Xylariales physiology
Xylariales ultrastructure
Random Amplified Polymorphic DNA Technique
Recombination, Genetic
Xylariales genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0172-8083
- Volume :
- 30
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 8753655
- Full Text :
- https://doi.org/10.1007/s002940050129