Back to Search
Start Over
Pretreatment with 17 beta-estradiol attenuates basal- and PTH-stimulated membrane adenylyl cyclase activity in human osteoblast-like SAOS-2 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1996 Aug 05; Vol. 225 (1), pp. 320-5. - Publication Year :
- 1996
-
Abstract
- Parathyroid hormone (PTH)-stimulated production of cAMP by intact human osteoblast-like SaOS-2 cells is diminished by pretreatment with 17 beta-estradiol (E2). The goal of the present study was to determine whether E2, affected adenylyl cyclase activity in cell membranes. Cells were deprived of steroid hormones for 24 h and then incubated with E2 (1 nM) or vehicle for 12 h. Cell membranes were prepared and incubated with [alpha-32P]-ATP in the absence or presence of agonist, and the amount of [32P]-cAMP produced was measured to quantify adenylyl cyclase activity. There was less cAMP produced by membranes from E2-treated cells in response to PTH, GTP gamma S, forskolin, and Mn2+. E2 had no effect on the amounts of Gs or Gi. We propose that pretreatment of SaOS-2 cells with E2 reduced membrane cyclase activity, at least in part, by actions on the PTH-sensitive adenylyl cyclase and/or the G protein-adenylyl cyclase complex.
- Subjects :
- Adenosine Triphosphate metabolism
Cell Line
Cell Membrane enzymology
Colforsin pharmacology
Cyclic AMP metabolism
Guanosine 5'-O-(3-Thiotriphosphate) pharmacology
Humans
Kinetics
Manganese pharmacology
Peptide Fragments pharmacology
Phosphorus Radioisotopes
Teriparatide
Time Factors
Adenylyl Cyclases metabolism
Estradiol pharmacology
Osteoblasts enzymology
Parathyroid Hormone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 225
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 8769137
- Full Text :
- https://doi.org/10.1006/bbrc.1996.1173