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Direct quantification of pentosidine in urine and serum by HPLC with column switching.
- Source :
-
Clinical chemistry [Clin Chem] 1996 Sep; Vol. 42 (9), pp. 1439-44. - Publication Year :
- 1996
-
Abstract
- Concentrations of pentosidine, an advanced glycation end product, are increased in aging, diabetes mellitus, and uremia. Using HPLC with column switching, we developed a direct method of measuring pentosidine in urine and serum. We inject the sample directly onto a gel-filtration precolumn, select ("heart-cut") the eluate fraction containing pentosidine, and introduce this fraction into a reversed-phased column by use of a switching valve. The recovery rate of the complete method was 97.7-99.9%. The intraassay CV was 5.7%, and the interassay CV was 5.8%. The calibration curve showed significant linearity (r = 0.998, P = 0.0001). We examined urinary concentrations of pentosidine in 12 diabetic patients (mean +/- SD, 8.7 +/- 2.3 micromol/mol of creatinine), 32 patients with chronic renal failure (CRF; 36.1 +/- 39.0), 19 osteoporotic patients (7.9 +/- 5.3), and 29 healthy control subjects (5.2 +/- 2.3). In CRF, urinary pentosidine in the patients undergoing hemodialysis was significantly higher than in CRF patients not being treated by hemodialysis (mean, 58.1 vs 18.2; P <0.001). Also, concentrations of urinary and serum pentosidine were significantly correlated (r = 0.797, P = 0.0011). Because this method does not require pretreatment of samples, it is convenient and useful for measuring urinary and serum pentosidine.
- Subjects :
- Adult
Aged
Aging
Arginine analysis
Arginine blood
Arginine urine
Diabetes Mellitus blood
Diabetes Mellitus urine
Humans
Kidney Failure, Chronic blood
Kidney Failure, Chronic urine
Lysine analysis
Lysine blood
Lysine urine
Middle Aged
Renal Dialysis
Time Factors
Uremia blood
Uremia urine
Arginine analogs & derivatives
Chromatography, High Pressure Liquid methods
Lysine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9147
- Volume :
- 42
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8787701