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High-level expression of active human plasminogen activator inhibitor type 1 (PAI-1) in E. coli.

Authors :
Zhou A
Pei Y
Wu H
Dong X
Xu X
Source :
Biochemistry and molecular biology international [Biochem Mol Biol Int] 1996 May; Vol. 39 (2), pp. 235-42.
Publication Year :
1996

Abstract

Plasminogen activator inhibitor 1 (PAI-1) is an important regulator of plasminogen activation, which inhibits both tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). The DNA sequence encoding mature PAI-1 protein was inserted into an inducible expression vector. This gene was highly expressed to produce a soluble active protein in E. coli cells. The amount of the recombinant protein was up to 20% of total cellular protein. By efficient purification with a yield of about 15-20%, the recombinant protein could be purified to homogeneity with its specific activity up to 6.1 x 10(4) (uPA) IU/mg. Its inhibitory activity declined during incubation at 37 degrees C with a half life of about 2 hr.

Details

Language :
English
ISSN :
1039-9712
Volume :
39
Issue :
2
Database :
MEDLINE
Journal :
Biochemistry and molecular biology international
Publication Type :
Academic Journal
Accession number :
8799449
Full Text :
https://doi.org/10.1080/15216549600201241