Back to Search
Start Over
The mouse inhibin alpha-subunit promoter directs SV40 T-antigen to Leydig cells in transgenic mice.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1996 May 31; Vol. 119 (2), pp. 135-46. - Publication Year :
- 1996
-
Abstract
- Testicular tumorigenesis was observed in transgenic mice expressing the 6-kb mouse inhibin alpha-subunit promoter/Simian virus 40 T-antigen (SV40 Tag) fusion gene. The tumors were confined to Leydig cells using immunohistochemistry with anti-Tag antibody, specific binding of biotinylated hCG and histochemistry for 3 beta-hydroxysteroid dehydrogenase. Leydig cell hyperplasia and presence of Tag protein in the testicular interstitial tissue were already evident at 5 and 6.5 days of age, respectively. An immortalized cell line, BLT-1, was established from one testicular tumor. These cells expressed the LH receptor and P450scc mRNAs, and displayed LH-responsive cAMP and progesterone production, and low testosterone production. The cells also specifically bound 125I-labeled recombinant human LH with high affinity (36000 binding sites/cell), and the binding was regulated by 8Br-cAMP and hCG. This gonadal tumor model is valuable for further studies on endocrine functions of Leydig cells and their tumorigenesis in vivo and in vitro.
- Subjects :
- 3-Hydroxysteroid Dehydrogenases metabolism
8-Bromo Cyclic Adenosine Monophosphate pharmacology
Animals
Antigens, Polyomavirus Transforming physiology
Cell Line, Transformed
Cholesterol Side-Chain Cleavage Enzyme genetics
Chorionic Gonadotropin metabolism
Chorionic Gonadotropin pharmacology
Cyclic AMP analysis
Humans
Hyperplasia
Leydig Cell Tumor pathology
Leydig Cell Tumor physiopathology
Leydig Cells pathology
Luteinizing Hormone metabolism
Male
Mice
Mice, Transgenic
Progesterone analysis
RNA, Messenger analysis
Receptors, LH analysis
Receptors, LH genetics
Testicular Neoplasms etiology
Testicular Neoplasms pathology
Testosterone analysis
Antigens, Polyomavirus Transforming genetics
Inhibins
Leydig Cell Tumor etiology
Peptides genetics
Promoter Regions, Genetic genetics
Simian virus 40 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 119
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 8807633
- Full Text :
- https://doi.org/10.1016/0303-7207(96)03802-6