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U14 snoRNAs are encoded in introns of human ribosomal protein S13 gene.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1996 Nov 12; Vol. 228 (2), pp. 371-4. - Publication Year :
- 1996
-
Abstract
- During the mapping and sequencing of human ribosomal protein genes, we have found that U14 small nucleolar RNAs (snoRNAs) are encoded in introns of the ribosomal protein S13 gene. PCR cloning and sequencing have been employed to obtain the intron-containing (functional) human S13 gene and to eliminate a number of the processed pseudogenes. The S13 gene consists of six exons and five introns and spans a genomic region of 3.3 kb. U14 snoRNA genes are positioned in introns 3 and 5 of the S13 gene. A sequence comparison of the U14 genes in human and Xenopus laevis revealed that the regions involved in binding to 18S rRNA and the consensus (boxes C and D) of sequences for snoRNAs are highly conserved among the genes in the two species.
- Subjects :
- Base Sequence
Conserved Sequence
DNA Primers
Exons
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 18S chemistry
RNA, Ribosomal, 18S genetics
RNA, Small Nuclear chemistry
RNA, Small Nuclear genetics
Restriction Mapping
Introns
RNA, Small Nuclear biosynthesis
Ribosomal Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 228
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 8920921
- Full Text :
- https://doi.org/10.1006/bbrc.1996.1668