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Dimethyl sulfoxide decreases interleukin-8-mediated neutrophil recruitment in the airways.
- Source :
-
The American journal of physiology [Am J Physiol] 1996 Nov; Vol. 271 (5 Pt 1), pp. L838-43. - Publication Year :
- 1996
-
Abstract
- In this study, we investigated the role of dimethyl sulfoxide (DMSO) in inhibiting interleukin-8 (IL-8)-mediated neutrophil recruitment induced by Pseudomonas aeruginosa (PA) bacterial supernatant. First, we tested whether DMSO could inhibit IL-8 production induced by PA in human bronchial epithelial (16-HBE) cells in vitro. In these cells, exposure to PA or H2O2 induced IL-8 production dose dependently, an effect that was inhibited by 1% DMSO at both the protein and RNA level. Second, we tested whether DMSO could block the recruitment of neutrophils induced by PA in a bypassed segment of dog trachea in vivo. PA supernatant was placed in the tracheal segment for 6 h in four dogs, and neutrophil recruitment and IL-8 concentrations were measured in the superfusate. DMSO prevented the recruitment of neutrophils and IL-8 production induced by PA time dependently. The results suggest that DMSO may play an anti-inflammatory role in the airway by inhibiting IL-8 production in epithelial cells.
- Subjects :
- Animals
Bronchi drug effects
Cell Line
Cells, Cultured
Dogs
Enzyme-Linked Immunosorbent Assay
Epithelium drug effects
Epithelium immunology
Epithelium physiology
Humans
Hydrogen Peroxide metabolism
Hydrogen Peroxide pharmacology
Interleukin-8 antagonists & inhibitors
Neutrophils drug effects
Protein Biosynthesis drug effects
Trachea drug effects
Trachea immunology
Transcription, Genetic drug effects
Bronchi physiology
Dimethyl Sulfoxide pharmacology
Gene Expression drug effects
Interleukin-8 biosynthesis
Neutrophils physiology
Pseudomonas aeruginosa immunology
Trachea physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 271
- Issue :
- 5 Pt 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 8944728
- Full Text :
- https://doi.org/10.1152/ajplung.1996.271.5.L838