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Expression of a new G protein-coupled receptor X-msr is associated with an endothelial lineage in Xenopus laevis.
- Source :
-
Mechanisms of development [Mech Dev] 1996 Oct; Vol. 59 (2), pp. 129-40. - Publication Year :
- 1996
-
Abstract
- In order to determine whether G protein-coupled receptors play a role in early embryogenesis, we looked for cDNA fragments amplified between primers located in consensus sequences of transmembrane segments. Using one such amplified fragment as a probe, we cloned a novel member of the G protein-coupled receptor superfamily in Xenopus. Alignment of the deduced protein sequence with that of other receptors discloses some homology with angiotensin receptors. A single transcript of 2.5 kb is detected at the late blastula stage and its expression increases during gastrulation. In situ hybridization reveals transcripts initially in the ventrolateral involuting marginal zone and later in the lateral plate mesoderm. At larval stages, the transcript is expressed in procardiac tube and forming blood vessels, where it is localized in the inner endothelial layer. Thus, this gene traces an endothelial lineage and represents a very early and unique marker in Xenopus of the specification of cardiac and vascular endothelia. We propose the name of X-msr for mesenchyme-associated serpentine receptor.
- Subjects :
- Amino Acid Sequence
Animals
Apelin Receptors
Base Sequence
Cloning, Molecular
Endothelium chemistry
Gene Expression Regulation, Developmental
In Situ Hybridization
Kinetics
Larva chemistry
Molecular Sequence Data
Receptors, Cell Surface genetics
Sequence Analysis, DNA
Receptors, Cell Surface chemistry
Receptors, G-Protein-Coupled
Xenopus Proteins
Xenopus laevis embryology
Subjects
Details
- Language :
- English
- ISSN :
- 0925-4773
- Volume :
- 59
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Mechanisms of development
- Publication Type :
- Academic Journal
- Accession number :
- 8951791
- Full Text :
- https://doi.org/10.1016/0925-4773(96)00585-0