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The tau protein in human cerebrospinal fluid in Alzheimer's disease consists of proteolytically derived fragments.
- Source :
-
Journal of neurochemistry [J Neurochem] 1997 Jan; Vol. 68 (1), pp. 430-3. - Publication Year :
- 1997
-
Abstract
- Previous studies have shown that the levels of the microtubule-associated protein tau in the CSF of patients with Alzheimer's disease (AD) are elevated compared with age-matched controls. In spite of these findings, the nature of tau in CSF has not been well documented. In the present study, tau was immunoprecipitated from CSF of patients with AD or acute stroke, as well as normal elderly controls, followed by immunoblot analysis. In all cases, CSF tau consisted primarily of a band migrating at 26-28 kDa. In AD and stroke patients, several smaller tau fragments were also detected. No intact tau was detected in any of the CSF samples examined. Further immunoprecipitation studies showed that the majority of the tau fragments contained the amino terminus of the molecule. Treatment of CSF tau with alkaline phosphatase did not alter the electrophoretic properties of the fragments. These studies clearly demonstrate that CSF tau is truncated rather than intact.
- Subjects :
- Cerebrovascular Disorders cerebrospinal fluid
Enzyme-Linked Immunosorbent Assay
Humans
Immunoblotting
Precipitin Tests
Reference Values
tau Proteins chemistry
Alzheimer Disease cerebrospinal fluid
Peptide Fragments cerebrospinal fluid
Peptide Hydrolases metabolism
tau Proteins cerebrospinal fluid
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 68
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8978756
- Full Text :
- https://doi.org/10.1046/j.1471-4159.1997.68010430.x