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Studies on the interaction of T-cells with major histocompatibility complex class II antigens.
- Source :
-
Clinical science (London, England : 1979) [Clin Sci (Lond)] 1997 Jan; Vol. 92 (1), pp. 25-36. - Publication Year :
- 1997
-
Abstract
- 1. Major histocompatibility complex class II antigens have the central role in the immune response of 'presenting' antigenic peptide to CD4+ T-cells. This interaction with a T-cell's receptor may result in activation, but, if recognition occurs without collateral molecular interactions which cause 'co-stimulation', these T-cells will be tolerized. 2. In the light of current interest in muscle cell transplantation, a transformed myoblast, TE671, phenotypically comparable to untransformed cells, transfected to express class II, was studied as a stable model of antigen presentation by muscle cells. These cells failed to activate T-cells but induced tolerance. 3. The DR alpha chain is unusual being the only non-polymorphic classical class II polypeptide, raising the question of its functional contribution. To this end, several single polypeptide constructs were generated with contributions from different class II alpha-chains. On this basis, it was established that DR alpha makes significant contributions to peptide binding and that its alpha 2 domain is also important in T-cell recognition, possibly through CD4 binding. 4. One implication of the lack of polymorphism of DR alpha may be that it has a wider range of pairing partners, possibly including beta chains of different isotypes. To address this, it is planned to use transfectants expressing only a mixed isotype pair to generate T-cell clones in vitro. These reagents would be useful tools to detect whether such mixed pairs exist physiologically. In this paper, the development of a system is described which will allow this question to be addressed.
- Subjects :
- CD4-Positive T-Lymphocytes immunology
Cell Line, Transformed
HLA-DR Antigens genetics
HLA-DR Antigens immunology
Humans
Immune Tolerance
Isomerism
Protein Binding
Antigen Presentation
Histocompatibility Antigens Class II immunology
Lymphocyte Activation
Muscle, Skeletal immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0143-5221
- Volume :
- 92
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinical science (London, England : 1979)
- Publication Type :
- Academic Journal
- Accession number :
- 9038588
- Full Text :
- https://doi.org/10.1042/cs0920025