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Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production.
- Source :
-
Molecular immunology [Mol Immunol] 1996 Oct; Vol. 33 (15), pp. 1157-64. - Publication Year :
- 1996
-
Abstract
- Incubation of human vitamin D3-binding protein (Gc protein), with a mixture of immobilized beta-galactosidase and sialidase, efficiently generated a potent macrophage activating factor, a protein with N-acetylgalactosamine as the remaining sugar. Stepwise incubation of Gc protein with immobilized beta-galactosidase and sialidase, and isolation of the intermediates with immobilized lectins, revealed that either sequence of hydrolysis of Gc glycoprotein by these glycosidases yields the macrophage-activating factor, implying that Gc protein carries a trisaccharide composed of N-acetylgalactosamine and dibranched galactose and sialic acid termini. A 3 hr incubation of mouse peritoneal macrophages with picomolar amounts of the enzymatically generated macrophage-activating factor (GcMAF) resulted in a greatly enhanced phagocytic activity. Administration of a minute amount (10-50 pg/mouse) of GcMAF resulted in a seven- to nine-fold enhanced phagocytic activity of macrophages. Injection of sheep red blood cells (SRBC) along with GcMAF into mice produced a large number of anti-SRBC antibody secreting splenic cells in 2-4 days.
- Subjects :
- Adjuvants, Immunologic physiology
Adoptive Transfer
Animals
Antibody Formation
Antibody-Producing Cells cytology
Cell Count
Erythrocytes
Female
Humans
Macrophage-Activating Factors pharmacology
Macrophages, Peritoneal immunology
Mice
Mice, Inbred BALB C
Neuraminidase pharmacology
Sheep blood
Time Factors
Vitamin D-Binding Protein drug effects
Vitamin D-Binding Protein isolation & purification
Vitamin D-Binding Protein pharmacology
beta-Galactosidase pharmacology
Macrophage-Activating Factors chemistry
Vitamin D-Binding Protein chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0161-5890
- Volume :
- 33
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9070663
- Full Text :
- https://doi.org/10.1016/s0161-5890(96)00081-8