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Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced by a withdrawal of NGF.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Feb 24; Vol. 231 (3), pp. 770-4. - Publication Year :
- 1997
-
Abstract
- We isolated mouse CPP32/apopain cDNA, a mammalian homologue most closely related to Ced-3 in C. elegans, and examined the involvement of CPP32 in the apoptosis of nervous system during development. CPP32 is specifically expressed in the trigeminal (V) ganglia, facio-acoustic (VII-VIII) ganglion complex, and dorsal root ganglia (DRGs) of mouse 10.5-day embryos. CPP32-like proteases are activated during apoptosis of DRG neurons induced by deprivation of NGF and serum. Ac-DEVD-CHO, an inhibitor for CPP32-like proteases, prevents apoptosis of DRG neurons, but Ac-YVAD-CHO, an inhibitor for ICE-like proteases, does not. These results suggest that CPP32 or CPP32-like proteases play a role as central mediator in the apoptosis of DRG neurons induced by lack of neurotrophin signals.
- Subjects :
- Amino Acid Sequence
Animals
Caspase 3
Cricetinae
Enzyme Activation
Ganglia, Spinal enzymology
Gene Expression Regulation
Humans
In Situ Hybridization
Mice
Molecular Sequence Data
RNA, Messenger genetics
Sequence Alignment
Sequence Homology, Amino Acid
Apoptosis drug effects
Caspases
Cysteine Endopeptidases genetics
Nerve Growth Factors pharmacology
Neurons, Afferent enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 231
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9070890
- Full Text :
- https://doi.org/10.1006/bbrc.1996.6002