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A fluorometric determination method for D,L configurations of per-O-methylated monosaccharides by anomeric 2-methyl-2-beta-naphthyl-1,3-benzodioxole 4-carboxylation and high-performance liquid chromatography.

Authors :
Bai C
Ohrui H
Nishida Y
Meguro H
Source :
Analytical biochemistry [Anal Biochem] 1997 Mar 15; Vol. 246 (2), pp. 246-52.
Publication Year :
1997

Abstract

Per-O-methylated pentopyranoses and hexopyranoses were converted to their glycosyl chlorides and coupled with cesium salt of fluorescent chiral derivatization reagent, (+)-2-methyl-2-beta-naphthyl-1,3-benzodioxole-4-carboxylic acid [(+)-MND carboxylic acid], to afford their 1-O-(+)-MNB carboxylates. The D,L enantiomers were separated by normal-phase HPLC and determined at the picomolar level, and this methodology could be extended to the highly sensitive simultaneous determination of per-O-methylated monosaccharides.

Details

Language :
English
ISSN :
0003-2697
Volume :
246
Issue :
2
Database :
MEDLINE
Journal :
Analytical biochemistry
Publication Type :
Academic Journal
Accession number :
9073363
Full Text :
https://doi.org/10.1006/abio.1997.2016