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A fluorometric determination method for D,L configurations of per-O-methylated monosaccharides by anomeric 2-methyl-2-beta-naphthyl-1,3-benzodioxole 4-carboxylation and high-performance liquid chromatography.
- Source :
-
Analytical biochemistry [Anal Biochem] 1997 Mar 15; Vol. 246 (2), pp. 246-52. - Publication Year :
- 1997
-
Abstract
- Per-O-methylated pentopyranoses and hexopyranoses were converted to their glycosyl chlorides and coupled with cesium salt of fluorescent chiral derivatization reagent, (+)-2-methyl-2-beta-naphthyl-1,3-benzodioxole-4-carboxylic acid [(+)-MND carboxylic acid], to afford their 1-O-(+)-MNB carboxylates. The D,L enantiomers were separated by normal-phase HPLC and determined at the picomolar level, and this methodology could be extended to the highly sensitive simultaneous determination of per-O-methylated monosaccharides.
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 246
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9073363
- Full Text :
- https://doi.org/10.1006/abio.1997.2016