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Protoporphyrin IX sensitized photohemolysis: stoichiometry of the reaction and repair by reduced glutathione.
- Source :
-
Physiological chemistry and physics [Physiol Chem Phys] 1977; Vol. 9 (1), pp. 63-74. - Publication Year :
- 1977
-
Abstract
- Protoporphyrin IX acts as a sensitizer in the photohemolysis of bovine erythrocytes by binding to a limited number of membrane sites. The cholesterol-specific antibiotic lucensomycin competes with protoporphyrin in binding to the membranes. The possibility of cholesterol peroxidation as a primary event in photohemolysis is supported by the repairing effect of exogenous cholesterol and by the increased susceptibility of the photosensitized erythrocytes to lucensomycin. Glutathione, if present within the erythrocyte, postpones the onset of lysis; if added after irradiation, it may repair the membrane damage and prevent hemolysis. This effect appears to be related to a redox reaction (possibly involving glutathione peroxidase) between reduced glutathione and the cholesterol peroxide molecules.
- Subjects :
- Animals
Cattle
Cholesterol
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Lucensomycin pharmacology
Membrane Lipids
Oxidation-Reduction
Peroxides
Photolysis
Glutathione pharmacology
Hemolysis drug effects
Light
Porphyrins radiation effects
Protoporphyrins radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 0031-9325
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Physiological chemistry and physics
- Publication Type :
- Academic Journal
- Accession number :
- 909954