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TPA induces transglutaminase C and inhibits cell growth in the colon carcinoma cell line SW620.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Mar 27; Vol. 232 (3), pp. 737-41. - Publication Year :
- 1997
-
Abstract
- In contrast to most other systems, TPA induced TGc activity and protein in SW620 human colon carcinoma cells. This induction was accompanied by cell growth inhibition and increased apoptosis. The general protein kinase-C inhibitor GF-109203X blocked the induction of TGc by TPA, whereas the specific inhibitor of the PKC alpha isoform, the indocarbazole Go6976, reduced it by 40%. These PKC inhibitors had similar inhibitory effects on TPA increased apoptosis and inhibition of cell growth, suggesting that the observed actions of TPA are mediated by PKC, and a close connection between TGc activity, increased apoptosis and cell growth inhibition. We conclude that TPA may offer new approaches in the management of colon cancer cell growth.
- Subjects :
- Apoptosis drug effects
Colonic Neoplasms pathology
Dose-Response Relationship, Drug
Enzyme Activation drug effects
Enzyme Induction drug effects
Humans
Protein Kinase C metabolism
Tetradecanoylphorbol Acetate administration & dosage
Thymidine metabolism
Tumor Cells, Cultured
Cell Division drug effects
Colonic Neoplasms drug therapy
Colonic Neoplasms enzymology
Tetradecanoylphorbol Acetate pharmacology
Transglutaminases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 232
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9126346
- Full Text :
- https://doi.org/10.1006/bbrc.1997.6363