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Preparation of single-celled marine dinoflagellates for electron microscopy.
- Source :
-
Microscopy research and technique [Microsc Res Tech] 1997 Feb 15; Vol. 36 (4), pp. 337-40. - Publication Year :
- 1997
-
Abstract
- Electron microscopy has been used successfully to study and identify single-celled marine dinoflagellates including parasitic ones and others, such as those that cause red tide. Delicate cells can be preserved for scanning electron microscopy with a combined glutaraldehydeosmium tetroxide mixture that is adjusted for the osmolality of the medium. The protocol allows resolution of fine morphological features. Preservation for transmission electron microscopy can be accomplished with a standard glutaraldehyde fixation and osmium-tetroxide post-fixation in a suitable buffer, but again, the osmolality of the mixture must be adjusted. The protocol allows ultrastructural resolution of vesiculated cells and has been modified for small sample sizes.
- Subjects :
- Animals
Microscopy, Electron
Osmolar Concentration
Dinoflagellida ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1059-910X
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microscopy research and technique
- Publication Type :
- Academic Journal
- Accession number :
- 9140933
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0029(19970215)36:4<337::AID-JEMT11>3.0.CO;2-Q