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Effect of exogenous nitric oxide and superoxide on interleukin-8 from human polymorphonuclear leucocytes.
- Source :
-
British journal of anaesthesia [Br J Anaesth] 1997 Jun; Vol. 78 (6), pp. 714-7. - Publication Year :
- 1997
-
Abstract
- Patients with acute inflammatory lung injury are commonly treated with inhaled nitric oxide. Nitric oxide has profound immunoregulatory effects. Increased concentrations of the cytokine interleukin-8 (IL-8) in bronchoalveolar lavage fluid has been associated with disease severity. We have investigated the effects of a nitric oxide donor and a combined nitric oxide-superoxide donor on lipopolysaccharide-mediated accumulation of IL-8 from cultured human neutrophils. Interleukin-8 was measured in culture supernatant after 20 h using enzyme immunoassay. The combined nitric oxide-superoxide donor, 3-morpholinosydnonimine (SIN-1), dose-dependently decreased lipopolysaccharide-mediated IL-8 accumulation (P < 0.01). SIN-1 also decreased IL-8 accumulation from unstimulated neutrophils (P < 0.001). In contrast, the pure nitric oxide donor, 1,2,3,4-oxatriazolium 5-amino chloride (GEA-3162), increased stimulated IL-8 accumulation (P < 0.01) and also increased IL-8 accumulation in unstimulated cells (P < 0.002). Nitric oxide and superoxide have profound effects on IL-8. These results have important implications for the treatment of patients with acute lung injury with inhaled nitric oxide.
- Subjects :
- Cell Culture Techniques
Dose-Response Relationship, Drug
Humans
Lipopolysaccharides immunology
Molsidomine analogs & derivatives
Molsidomine pharmacology
Neutrophil Activation
Neutrophils immunology
Platelet Aggregation Inhibitors pharmacology
Triazoles pharmacology
Interleukin-8 blood
Neutrophils drug effects
Nitric Oxide pharmacology
Superoxides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0007-0912
- Volume :
- 78
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- British journal of anaesthesia
- Publication Type :
- Academic Journal
- Accession number :
- 9215025
- Full Text :
- https://doi.org/10.1093/bja/78.6.714