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Rapid miniaturized chromatography for technetium-99m-tetrofosmin.
Rapid miniaturized chromatography for technetium-99m-tetrofosmin.
- Source :
-
Journal of nuclear medicine technology [J Nucl Med Technol] 1997 Mar; Vol. 25 (1), pp. 52-4. - Publication Year :
- 1997
-
Abstract
- Unlabelled: The purpose of this investigation was to develop and evaluate a new and rapid miniaturized chromatography system that would accurately assess the radiochemical purity of 99mTc-tetrofosmin without the problems of solvent ratios and time requirements associated with the manufacturer's recommended procedure.<br />Methods: The migration of the radiochemical components of 99mTc-tetrofosmin was evaluated using various chromatography media with ethyl acetate as the solvent. After optimization of the miniaturized system, radiochemical purity assessments were performed simultaneously on 23 99mTc-tetrofosmin preparations using both recommended and miniaturized chromatography systems.<br />Results: A miniaturized chromatography system consisting of Whatman 1 chromatography paper with ethyl acetate was developed for the radiochemical purity assessment of 99mTc-tetrofosmin. Radiochemical purity results for 99mTc-tetrofosmin preparations were similar with both recommended and miniaturized chromatography methods, with a mean difference of 1.5% +/- 1.2% (s.d.). Differences in radiochemical purity results between the two chromatography systems were less than 2% (20 of 23 evaluations) with most preparations.<br />Conclusion: Radiochemical purity results for 99mTc-tetrofosmin preparations were similar with both the manufacturer's recommended chromatography and miniaturized chromatography systems. The miniaturized chromatography system is easier to use, and the time required to perform radiochemical purity assessments is substantially reduced.
- Subjects :
- Acetates chemistry
Microchemistry
Micromanipulation
Miniaturization
Organophosphorus Compounds isolation & purification
Organotechnetium Compounds isolation & purification
Paper
Quality Control
Radiochemistry
Radiopharmaceuticals isolation & purification
Sodium Pertechnetate Tc 99m analysis
Solvents chemistry
Technetium analysis
Time Factors
Chromatography, Paper methods
Organophosphorus Compounds analysis
Organotechnetium Compounds analysis
Radiopharmaceuticals analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0091-4916
- Volume :
- 25
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of nuclear medicine technology
- Publication Type :
- Academic Journal
- Accession number :
- 9239605