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Neutralization of bothropic and crotalic venom toxic activities by IgG(T) and IgGa subclasses isolated from immune horse serum.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 1997 Jun; Vol. 35 (6), pp. 931-6. - Publication Year :
- 1997
-
Abstract
- IgG(T) and IgGa isotypes were isolated from horse hyperimmune anti-bothropic and anti-crotalic sera using a combination of two affinity chromatographic processes. IgG(T) and IgGa isotypes were isolated from these sera by chromatography on protein A-Sepharose followed by separation of the two isotypes by chromatography on a column of anti-IgG(T)-Sepharose. LO-HoGT-1, a rat anti-horse IgG(T) monoclonal antibody, was used. A comparative study of the efficiency of these isotypes in neutralizing the main toxic activities of the homologous venoms was carried out. It was found that IgG(T) was about three-fold and seven-fold more protective than IgGa for neutralization of the lethal activity of B. jararaca and C. d. terrificus venoms, respectively. IgG(T) was also more effective than IgGa for the neutralization of the haemorrhagic activity induced by B. jararaca venom, while both isotypes neutralized equally well the blood incoagulability induced by this venom. The results suggest that IgG(T) is the most protective isotype present in both anti-bothropic and anti-crotalic sera, followed by IgGa. Owing to their very low concentration in the serum, other IgG isotypes are not likely to be important in neutralizing the venoms' toxic activities.
- Subjects :
- Animals
Antibody Specificity
Blood Coagulation Disorders immunology
Crotalid Venoms toxicity
Hemorrhage chemically induced
Hemorrhage immunology
Humans
Immune Sera
Male
Mice
Viper Venoms toxicity
Bothrops
Crotalid Venoms immunology
Horses immunology
Immunoglobulin G immunology
Viper Venoms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0041-0101
- Volume :
- 35
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 9241786
- Full Text :
- https://doi.org/10.1016/s0041-0101(96)00177-8