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Experimental bilateral urinary obstruction in fetal sheep: transforming growth factor-beta 1 expression.
- Source :
-
The American journal of physiology [Am J Physiol] 1997 Sep; Vol. 273 (3 Pt 2), pp. F372-9. - Publication Year :
- 1997
-
Abstract
- To gain insight into the role of transforming growth factor-beta 1 (TGF-beta 1) in the development of kidney pathology following fetal obstruction, we measured TGF-beta 1 gene expression, the active peptide, and the urinary concentration in a model of fetal bilateral urinary obstruction (BUO) in sheep. Fetal lambs underwent BUO at 60 (FO-60) or 80 days (FO-80) of gestation and were studied at 120 days. Independently of the onset or duration of obstruction, all fetuses developed type IV dysplasia (IV) associated with an arrest in the nephrogenesis or hydronephrosis. Fetal glomerular filtration rate was not significantly modified, whereas sodium tubular reabsorption was significantly decreased, and urinary TGF-beta 1 concentration was elevated in hydronephrosis but not in IV. Levels of TGF-beta 1 mRNA were increased in hydronephrosis compared with normal kidneys, and active TGF-beta 1 immunoreactivity was increased in both hydronephrotic and IV kidneys. In summary, TGF-beta 1 may play a role in the development of hydronephrosis and dysplasia in kidneys following fetal BUO. Its role in the arrest of nephrogenesis observed in the IV kidneys remains to be proved.
- Subjects :
- Animals
Female
Gene Expression Regulation, Developmental
Gestational Age
Hydronephrosis embryology
Hydronephrosis pathology
Kidney cytology
Kidney pathology
Kidney Glomerulus cytology
Kidney Glomerulus embryology
Kidney Glomerulus pathology
Pregnancy
Sheep
Transforming Growth Factor beta urine
Urethral Obstruction pathology
Urethral Obstruction physiopathology
Kidney embryology
Transforming Growth Factor beta biosynthesis
Urethral Obstruction embryology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 273
- Issue :
- 3 Pt 2
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 9321909
- Full Text :
- https://doi.org/10.1152/ajprenal.1997.273.3.F372