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Gene expression of the insulin-like growth factor system during mouse kidney development.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1997 Sep 19; Vol. 132 (1-2), pp. 81-91. - Publication Year :
- 1997
-
Abstract
- Expression of the insulin-like growth factor (IGF) system was investigated in mouse renal development and physiology, using non radioactive in situ hybridization and quantitative RT-PCR. IGF-I mRNA levels increased after birth and were confined to distal tubules and peritubular capillaries in the outer medulla. IGF-II mRNA levels were high in developing kidneys and peaked after birth. The type I receptor mRNA expression pattern mostly parallelled those of IGF-I and IGF-II. The IGF binding proteins (IGFBP's) showed weak mRNA expression for IGFBP-1 and -6. High fetal mRNA levels were measured for IGFBP-2, showing a similar profile in time as observed for IGF-II. Low fetal IGFBP-3 and -5 mRNA levels increased after birth. IGFBP-2, -4 and -5 mRNA expression was localized to differentiating cells. In the mature kidney predominant expression was confined to proximal tubules (IGFBP-4), thin limbs of Henle's Loop (IGFBP-2), glomerular mesangial cells (IGFBP-5) and peritubular capillaries of the medulla (IGFBP-5). IGFBP-3 mRNA was exclusively expressed in endothelial cells of the renal capillary system. Distinct mRNA expression for each member of the IGF system may point to specific roles in development and physiology of the mouse kidney.
- Subjects :
- Animals
In Situ Hybridization
Insulin-Like Growth Factor Binding Protein 1 biosynthesis
Insulin-Like Growth Factor Binding Protein 2 biosynthesis
Insulin-Like Growth Factor Binding Protein 4 biosynthesis
Insulin-Like Growth Factor I biosynthesis
Insulin-Like Growth Factor II biosynthesis
Kidney embryology
Mice
Mice, Inbred BALB C
Polymerase Chain Reaction
RNA, Messenger analysis
RNA, Messenger biosynthesis
RNA, Messenger genetics
Gene Expression Regulation, Developmental
Insulin-Like Growth Factor Binding Protein 1 genetics
Insulin-Like Growth Factor Binding Protein 2 genetics
Insulin-Like Growth Factor Binding Protein 4 genetics
Insulin-Like Growth Factor I genetics
Insulin-Like Growth Factor II genetics
Kidney metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 132
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 9324049
- Full Text :
- https://doi.org/10.1016/s0303-7207(97)00123-8