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Dexamethasone suppresses apoptosis in a human gastric cancer cell line through modulation of bcl-x gene expression.
- Source :
-
FEBS letters [FEBS Lett] 1997 Sep 22; Vol. 415 (1), pp. 11-5. - Publication Year :
- 1997
-
Abstract
- Treatment of human gastric cancer TMK-1 cells with transcription and translation inhibitors rapidly triggered cell apoptosis. Along with cell apoptosis, the Bcl-xS level was markedly upregulated suggesting a crucial role of this protein in promoting the apoptotic process. In the presence of dexamethasone, however, cell apoptosis was greatly attenuated as demonstrated by DNA histogram shift and DNA fragmentation. Studies using the glucocorticoid receptor antagonist RU486 indicated that attenuation of apoptosis was mediated through glucocorticoid receptors. Dexamethasone not only suppressed the apoptosis-associated upregulation of Bcl-xS but also enhanced the basal level of Bcl-xL in the cells. In addition, bcl-x mRNA stability was significantly extended in the presence of dexamethasone. These results indicate that dexamethasone exerted a protective effect and delayed apoptosis of TMK-1 cells by modulating bcl-x gene expression.
- Subjects :
- Cycloheximide pharmacology
DNA Fragmentation drug effects
Dactinomycin pharmacology
Dichlororibofuranosylbenzimidazole pharmacology
Flow Cytometry
Humans
Immunoblotting
Mifepristone pharmacology
Nucleic Acid Synthesis Inhibitors pharmacology
Protein Synthesis Inhibitors pharmacology
Proto-Oncogene Proteins c-bcl-2 metabolism
RNA, Messenger analysis
RNA, Messenger metabolism
Receptors, Glucocorticoid antagonists & inhibitors
Receptors, Glucocorticoid metabolism
Stomach Neoplasms
Tumor Cells, Cultured
Up-Regulation drug effects
bcl-X Protein
Apoptosis drug effects
Dexamethasone pharmacology
Gene Expression Regulation, Neoplastic drug effects
Proto-Oncogene Proteins c-bcl-2 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 415
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 9326359
- Full Text :
- https://doi.org/10.1016/s0014-5793(97)01083-1