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Integration of Langerhans cells into a pigmented reconstructed human epidermis.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1997 Oct; Vol. 109 (4), pp. 510-2. - Publication Year :
- 1997
-
Abstract
- The majority of in vitro reconstructed human epidermis is composed of keratinocytes only. Recently, the introduction of melanocytes into epidermal reconstructs has enlarged their field of application. The completion of reconstructed epidermis by introducing Langerhans cells remained an important challenge because Langerhans cells, unlike the other epidermal cell types, cannot be subcultured and expanded. To solve this problem, we used cord blood-derived CD34+ hematopoietic progenitors. Seeding these cells, after induction of their differentiation by granulocyte macrophage-colony stimulating factor and tumor necrosis factor-alpha, onto a reconstructing epidermis, composed of keratinocytes and melanocytes, gives rise to a pigmented epidermis with melanocytes in the basal layer and resident epidermal Langerhans cells located suprabasally. Interestingly, the same result was obtained by co-seeding a mixture of keratinocytes, melanocytes, and nondifferentiated CD34+ hematopoietic progenitors on the dermal equivalent, indicating that keratinocytes provide the environmental conditions for hematopoietic progenitors to differentiate into resident epidermal Langerhans cells, expressing major histocompatibility complex class II molecules, CD1a antigen, and Birbeck granules.
- Subjects :
- Antigens, CD34 analysis
Cell Differentiation
Cells, Cultured
Cytological Techniques
Epidermal Cells
Fetal Blood cytology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Hematopoietic Stem Cells drug effects
Hematopoietic Stem Cells immunology
Hematopoietic Stem Cells physiology
Humans
Keratinocytes cytology
Melanocytes cytology
Microscopy, Electron
Tumor Necrosis Factor-alpha pharmacology
Epidermis physiology
Langerhans Cells physiology
Pigmentation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 109
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 9326382
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12336627