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Regulatory roles of high-density and low-density lipoproteins in cellular proliferation and secretion of progesterone and insulin-like growth factor I by enriched cultures of bovine small and large luteal cells.
- Source :
-
Journal of animal science [J Anim Sci] 1997 Dec; Vol. 75 (12), pp. 3235-45. - Publication Year :
- 1997
-
Abstract
- We tested the hypotheses that bovine high-density (HDL) and low-density (LDL) lipoproteins differentially influence cellular proliferation and progesterone and IGF-I production by bovine small and large luteal cells. Unit gravity sedimentation was used to produce enriched cultures of small (> 95% pure) and large (75 to 90% pure) luteal cells from corpora lutea (CL) on d 4 and 10 of the estrous cycle. Addition of LDL, HDL, or both resulted in the maintenance of higher (P < .05) numbers of 3beta-hydroxysteroid dehydrogenase (HSD)-positive small and large cells in culture and produced a marked proliferation of 3beta-HSD-negative small luteal cells compared to control medium. Low-density lipoprotein and HDL each stimulated greater (P < .01) progesterone secretion in enriched large cell cultures on both days of the cycle, and by small luteal cells on d 10, compared to the control. Together, LDL and HDL maximized this response. Lipoproteins markedly stimulated (P < .01) the secretion of IGF-I by bovine large luteal cells, and secretion was greater (P < .05) by cells from d 10 CL compared to d 4 CL. Results suggest that the actions of lipoproteins in bovine luteal cells extend beyond their widely recognized roles in steroidogenesis and include remarkable effects on cellular proliferation and IGF-I secretion. Type of lipoprotein (LDL vs HDL) did not have differential effects on any of the variables measured.
- Subjects :
- 3-Hydroxysteroid Dehydrogenases analysis
Animals
Cattle metabolism
Cell Division drug effects
Cell Division physiology
Cells, Cultured
Corpus Luteum chemistry
Female
Lipoproteins, HDL physiology
Lipoproteins, LDL physiology
Luteinizing Hormone pharmacology
Radioimmunoassay veterinary
Time Factors
Cattle physiology
Corpus Luteum cytology
Corpus Luteum metabolism
Insulin-Like Growth Factor I metabolism
Lipoproteins, HDL pharmacology
Lipoproteins, LDL pharmacology
Progesterone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8812
- Volume :
- 75
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of animal science
- Publication Type :
- Academic Journal
- Accession number :
- 9419998
- Full Text :
- https://doi.org/10.2527/1997.75123235x