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A defective interference-like phenomenon of human hepatitis B virus in chronic carriers.
- Source :
-
Journal of virology [J Virol] 1998 Jan; Vol. 72 (1), pp. 578-84. - Publication Year :
- 1998
-
Abstract
- Defective interfering (DI) particles have been found in many RNA and DNA viruses of bacteria, plants, and animals since their first discovery in influenza virus. However, this fundamental phenomenon has not been demonstrated in human natural infections. Using a new approach, here we provide the first experimental evidence for the existence of DI-like viruses in human chronic carriers of hepatitis B virus (HBV). Functional characterization of naturally occurring core internal deletion (CID) variants of HBV revealed all of the features of DI particles. When equal amounts of wild-type and CID variant DNAs were cotransfected into a human hepatoma cell line, Huh7, a three- to fivefold enrichment of CID variants was most often observed. The fluctuations of the virus populations between CID variants and helper HBV in three chronic carriers are reminiscent of the cycling phenomenon in other DI viral systems. This finding has important implications for chronic viral hepatitis and other chronic progressive viral diseases.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cell Line
DNA Primers genetics
Defective Viruses genetics
Genetic Variation
Hepatitis B virus genetics
Humans
Molecular Probe Techniques
Molecular Sequence Data
Phenotype
Polymerase Chain Reaction
Transfection
Viral Interference
Carrier State virology
Defective Viruses isolation & purification
Defective Viruses pathogenicity
Hepatitis B virology
Hepatitis B virus isolation & purification
Hepatitis B virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 72
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 9420261
- Full Text :
- https://doi.org/10.1128/JVI.72.1.578-584.1998