DNA fingerprinting of Mycobacterium tuberculosis complex culture isolates collected in Brazil and spotted onto filter paper.

Authors :: Burger M
Raskin S
Brockelt SR
Amthor B
Geiss HK
Haas WH
Source :: Journal of clinical microbiology [J Clin Microbiol] 1998 Feb; Vol. 36 (2), pp. 573-6.
Publication Year :: 1998
Abstract: The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens analyzed by an amplification-based typing method provide a new resource for epidemiological studies of infectious diseases.

Subjects :: Brazil epidemiology
DNA Fingerprinting
DNA, Bacterial genetics
DNA, Bacterial metabolism
Humans
Molecular Epidemiology
Mycobacterium tuberculosis genetics
Paper
Phylogeny
Tuberculosis epidemiology
DNA, Bacterial analysis
Mycobacterium tuberculosis isolation & purification
Polymerase Chain Reaction methods
Specimen Handling
Tuberculosis diagnosis

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