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Altered adherence properties of a Streptococcus gordonii hppA (oligopeptide permease) mutant result from transcriptional effects on cshA adhesin gene expression.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 1998 Jan; Vol. 144 ( Pt 1), pp. 127-136. - Publication Year :
- 1998
-
Abstract
- Cell-surface polypeptide CshA (259 kDa) mediates multiple adherence interactions of Streptococcus gordonii. By generating a chromosomal cshA promoter (p-cshA)-cat gene fusion and measuring both CAT enzyme activity and cat mRNA levels, it was shown that cshA is expressed maximally in cells in the late exponential phase of growth in batch culture. The expression of CAT enzyme activity from the p-cshA-cat promoter fusion was 28% decreased in early stationary phase cell extracts of mutant strain OB528 in which the hppA (oligopeptide-binding lipoprotein) gene was insertionally inactivated. This effect was correlated with proportionally reduced cell-surface expression of CshA protein and with impaired adherence of hppA mutant cells to cells of an oral Actinomyces naeslundii strain. cshA promoter activity was enhanced in streptococcal cells that were incubated in conditioned culture medium as opposed to fresh medium, but this did not occur in an hppA genetic background. It is suggested that HppA is necessary for the response of cells to an extracellular factor that modulates cshA transcription, and hence affects cell-surface CshA expression and streptococcal cell adherence properties.
- Subjects :
- ATP-Binding Cassette Transporters genetics
ATP-Binding Cassette Transporters metabolism
Base Sequence
Chloramphenicol O-Acetyltransferase
Cloning, Molecular
Genotype
Kinetics
Molecular Sequence Data
Recombinant Fusion Proteins biosynthesis
Streptococcus genetics
Transcription, Genetic
Bacterial Adhesion genetics
Bacterial Proteins biosynthesis
Carrier Proteins genetics
Carrier Proteins metabolism
Gene Expression Regulation, Bacterial
Lipoproteins genetics
Lipoproteins metabolism
Membrane Proteins
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Promoter Regions, Genetic
Streptococcus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1350-0872
- Volume :
- 144 ( Pt 1)
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 9467905
- Full Text :
- https://doi.org/10.1099/00221287-144-1-127