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Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1998 Mar 01; Vol. 26 (5), pp. 1179-84. - Publication Year :
- 1998
-
Abstract
- The yeast and human RAD51 genes encode strand-transfer proteins that are thought to be involved in both recombinational repair of DNA damage and meiotic recombination. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, five genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1), and here we report the isolation of the sixth member, RAD51C. RAD51C was originally identified by a computer screen of the EST database. A full-length approximately 1.3 kb cDNA clone has been isolated that encodes a protein of 376 aa, having a 18-26% aa identity with other human Rad51 family members. RAD51C includes a previously mapped sequenced-tagged site location near the end of chromosome 17q. The RAD51C transcript is expressed in various human tissues, with highest level of expression in testis, followed by heart muscle, spleen and prostate. Yeast two-hybrid experiments indicate that the Rad51C protein binds to two other members of the Rad51 protein family (Xrcc3 and Rad51B) but not to itself. These findings suggest that Rad51C may function similarly to the yeast Rad55 or Rad57 proteins, rather than as a Rad51 functional homolog.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Repair genetics
DNA, Complementary genetics
Fungal Proteins genetics
Gene Expression
Genes, Fungal
Humans
Molecular Sequence Data
Rad51 Recombinase
Recombination, Genetic
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Tissue Distribution
DNA-Binding Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 9469824
- Full Text :
- https://doi.org/10.1093/nar/26.5.1179